First Author:Jifan Li
Correspondence Author:Hongbin Sun, Qionglin Liang
Co author:Lei Liu, Yongjian Ai, Yang Liu
Journal:Acs Applied Materials & Interfaces
Issue:5
Volume:12
Impact Factor:8.3
DOI number:10.1021/acsami.9b19161
Affiliation of Author(s):Department of Chemistry, Northeastern University
Teaching and Research Group:物理化学
Place of Publication:UNITED STATES
Abstract:Fabrication of functional electrochemical biosensor is a hot topic; however, precise and sensitive cancer detection in early clinical diagnosis is still a great challenge. Continuous efforts have been devoted to explore functional materials for this issue. In this work, we developed a dual binding sites and dual signal amplifying electrochemical aptasensor of self-polymerized dopamine-decorated Au and coordinated with Fe-MOF (Au@PDA@Fe-MOF) for the detection of carcinoembryonic antigen (CEA). Remarkably, Au@PDA@Fe-MOF features high sensitivity, multiple active sites, good biocompatibility, and excellent selectivity, which is attributed to abundant -COOH in porous Fe-MOF and unsaturated Fe3+ sites on the surface of Fe-MOF as the active binding sites grafting more NH2-functionalized CEA-specific aptamer and redox PDA and Fe-MOF accelerating the movement of electrons for dual signal amplifying. Meanwhile, the electrochemical aptasensor shows favorable repeatability with 1.82% relative standard deviation (RSD) under five independent aptasensors and strong stability with only 3.3% degradation after 12 days of storage. In addition, the aptasensor has wide CEA detection range from 1 fg mL(-1) to 1 mu g mL(-1) with a low detection limit of 0.33 fg mL(-1) (S/N = 3). Furthermore, the aptasensor is feasible for accurate and quantitative detection of CEA in serum samples with RSD below 2.32%. The satisfying results demonstrate promising applications of the CEA aptasensor in practical sample analysis and lay an important foundation for other biomarker
Key Words:carcinoembryonic antigen, electrochemical aptasensor, Au@PDA@Fe-MOF, dual binding sites, dual signal amplifying
Document Code:WOS:000512216900031
Discipline:Natural Science
First-Level Discipline:Chemistry
Page Number:5500-5510
ISSN No.:1944-8244
Translation or Not:no