第一作者：Jifan Li

通讯作者：Hongbin Sun, Qionglin Liang

合写作者：Lei Liu, Yongjian Ai, Yang Liu

发表刊物：Acs Applied Materials & Interfaces

期号：5

卷号：12

影响因子：8.3

DOI码：10.1021/acsami.9b19161

所属单位：Department of Chemistry, Northeastern University

教研室：物理化学

刊物所在地：UNITED STATES

摘要：Fabrication of functional electrochemical biosensor is a hot topic; however, precise and sensitive cancer detection in early clinical diagnosis is still a great challenge. Continuous efforts have been devoted to explore functional materials for this issue. In this work, we developed a dual binding sites and dual signal amplifying electrochemical aptasensor of self-polymerized dopamine-decorated Au and coordinated with Fe-MOF (Au@PDA@Fe-MOF) for the detection of carcinoembryonic antigen (CEA). Remarkably, Au@PDA@Fe-MOF features high sensitivity, multiple active sites, good biocompatibility, and excellent selectivity, which is attributed to abundant -COOH in porous Fe-MOF and unsaturated Fe3+ sites on the surface of Fe-MOF as the active binding sites grafting more NH2-functionalized CEA-specific aptamer and redox PDA and Fe-MOF accelerating the movement of electrons for dual signal amplifying. Meanwhile, the electrochemical aptasensor shows favorable repeatability with 1.82% relative standard deviation (RSD) under five independent aptasensors and strong stability with only 3.3% degradation after 12 days of storage. In addition, the aptasensor has wide CEA detection range from 1 fg mL(-1) to 1 mu g mL(-1) with a low detection limit of 0.33 fg mL(-1) (S/N = 3). Furthermore, the aptasensor is feasible for accurate and quantitative detection of CEA in serum samples with RSD below 2.32%. The satisfying results demonstrate promising applications of the CEA aptasensor in practical sample analysis and lay an important foundation for other biomarker

关键字：carcinoembryonic antigen, electrochemical aptasensor, Au@PDA@Fe-MOF, dual binding sites, dual signal amplifying

论文编号：WOS:000512216900031

学科门类：理学

一级学科：化学

页面范围：5500-5510

ISSN号：1944-8244

是否译文：否